Detection of human papillomavirus DNA by the hybrid capture assay.

Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers.

The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R pri...

Comparison of the AdvanSure Human Papillomavirus Screening Real-Time PCR, the Abbott RealTime High Risk Human Papillomavirus Test, and the Hybrid Capture Human Papillomavirus DNA Test for the Detection of Human Papillomavirus

BACKGROUND We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2). METHODS All 3 tests were performed on 177 samples, and any samp...

Hybrid capture II, a new sensitive test for human papillomavirus detection. Comparison with hybrid capture I and PCR results in cervical lesions.

AIM To test a new assay for the detection of human papillomavirus (HPV) DNA, hybrid capture II (HC II), compared with the previous commercialized hybrid capture I (HC I) and polymerase chain reaction (PCR) results on cervical scrapes from fresh cone excision biopsy samples. METHODS The three methods were used on cervical scrapes from 42 fresh cone excision biopsy samples. There were nine meta...

Hybrid Capture 2 High-Risk HPV DNA TestTM An In Vitro Nucleic Acid Hybridization Assay with Signal Amplification using Microplate Chemiluminescence for the Qualitative Detection of Human Papillomavirus (HPV) Types

Detection of Human Papillomavirus DNA by PCR in Esophageal Squamous Cell Carcinoma from Turkmen Sahra, North-East of Iran

Human papillomavirus (HPV) DNA has been identified in esophageal carcinomas. However, the incidence of HPV varies significantly in different geographical locations. In this study, neoplasms from Turkmen Sahra, a region in Golestan province in northeast part of Iran, with a high incidence of squamous cell carcinoma were analyzed for the presence of HPV DNA. Turkmen Sahra is located in the cancer...

No Evidence for Human Papillomavirus in Patients with HIV in Iran

Background: Human Papillomaviruses (HPVs) have anestablished role in the development of cervical cancer. However, the presence of underlying conditions such as HIV/AIDS is necessary for this to occur. This studyaimed to evaluate the prevalence of HPV DNA in plasma samples from HIV-positive patients in Tehran, Iran. Methods: Plasma specimens from 95 patients diagnosed with HIV infection from Te...